teemi.build.transformation

This part of the lab module is used for making transformations

Functions

ODtime(initialOD, time[, td])

Calculates the OD based on doupling time.

calculate_volume_and_total_concentration(…)

Calculates the volume and total concentration of a list of DNA parts.

ng_to_nmol(ng, bp)

Calculates nanogram to nanomol for transformation mixes.

pool_parts(amplicons, part_names, …)

Pools amplicon parts and returns a dictionary of pooled volumes.

print_pooled_parts(pooled_volumes)

Prints the pooled parts and calculated concentrations.

time_to_inoculate(initialOD, td, verbose, …)

Calculates when a starter culture is ready to be transformed.

transformation_mix(reaction_names, …[, media])

Create a pandas DataFrame for the parts needed in transformation mixes.

transformation_partitipants(…[, amnt, …])

Returns a dict with the µl amounts needed in a transformation reaction.

wanted_mass(wanted_moles, size)

Calculates the mass needed from the specified amount of moles and size.

wanted_volume(wanted_mass, actual_concentration)

Calculates the wanted volume from the mass and concentration.